Regulatory

Part:BBa_K2708002:Design

Designed by: LAN FEI   Group: iGEM18_BIT   (2018-10-02)


Promoter with an Riboswitch that responds to S-adenosyl methionine molecules.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid is designed to verify the function of the SAM riboswitch.


Source

Synthesis

Riboswitch is a kind of RNA sequence element with special spatial structure. It is mainly found in the non-coding region of bacterial mRNA. SAM riboswitch is widely distributed in various bacterial lineages.

References